Figure 5

Figure 5

AP2M1 activates the Notch pathway. A) The enrichment score of stemness‐related pathways, Notch, Wnt, TGFβ, and Hedgehog signaling pathways in AP2M1+ malignant HSPCs from scRNA‐seq data. The significance is as follows, ^*p < .05, ^**p < 0.01, ^***p < .001. B) The enrichment score of the Notch signaling pathway according to cell malignancy and AP2M1 expression in HSPCs from scRNA‐seq data. C) The correlation between AP2M1 expression and the Notch signaling gene set expression in HSPCs from scRNA‐seq data. The density plots above the horizontal and vertical axes illustrate the distribution of AP2M1 and Notch pathway gene set expression, respectively. The correlation coefficients and p‐values are described at the top left. D) The protein levels of genes involved in the Notch1 signaling pathway. The proteins are assessed in both control and AP2M1‐overexpressing cell lines. E) The mRNA expression levels of Notch1 pathway genes in control and AML patient samples. F) Correlation between AP2M1 and Notch pathway genes in normal and malignant HSPCs from scRNA‐seq dataset. Only the significant correlation coefficients are shown. G) Fluorescence imaging analysis of Tp1 expression in Tp1, GFP zebrafish embryos. The knockdown zebrafish embryos by injection of ap2m1a‐MO and un‐injected control zebrafish embryos are shown. H) qPCR analysis of relative expression of Notch signaling pathway genes in zebrafish embryos with ap2m1a‐MO knockdown. I) mRNA expression levels of notch1a and notch1b in response to ap2m1a overexpression. 900 pg nL⁻¹ of ap2m1a mRNA was injected into zebrafish embryos. J,K) qPCR analysis of notch1a and notch1b mRNA expression level in zebrafish embryos injected at the one‐cell stage with AML1‐ETO mRNA alone or co‐injected with AML1‐ETO and AP2M1 mRNAs. Embryos were treated with idarubicin (10 nm) from 24 to 72 hpf, and notch1a and notch1b mRNA expression was compared between idarubicin‐treated and untreated groups within each injection condition.