Figure 1

Figure 1

AP2M1a is associated with the emergence of hematopoietic stem cells. A) TSNE plot of scRNA‐seq data derived from normal zebrafish. The color of each dot represents the corresponding cell identity. B) Expression level of ap2m1 in normal zebrafish. The dots within each violin plot represent the average expression values. C) WISH analysis and lateral view imaging of ap2m1a expression pattern in 24 hpf zebrafish embryos. D) WISH analysis and lateral view imaging of runx1 (26 hpf) and cmyb (36 hpf) expression in zebrafish embryos knockdown by injection of ap2m1a morpholino (ap2m1a‐MO) and un‐injected control zebrafish embryos. E) Fluorescence imaging analysis of cmyb and fli1 expression in the dorsal aorta of 48 hpf zebrafish embryos knocked down by injection of ap2m1a‐MO, and in un‐injected control zebrafish embryos. F) Number of double‐positive cells for cmyb and fli1 identified in 48 hpf zebrafish embryos knocked down by injection of ap2m1a‐MO, and un‐injected control zebrafish embryos. G) WISH analysis and lateral view imaging of cmyb expression in the CHT 3 dpf WT zebrafish embryos knocked down by injection of ap2m1a‐MO and un‐injected control zebrafish embryos. Brackets indicate HSPCs at CHT. H) Confocal imaging analysis and lateral view images of CD41 expression in the CHT 3 dpf CD41, GFP zebrafish embryos knocked down by injection of ap2m1a‐MO and un‐injected control zebrafish embryos. I) Quantification of CD41‐positive cells in 3 dpf CD41, GFP zebrafish embryos knocked down by injection of ap2m1a‐MO and un‐injected control zebrafish embryos.