Figure 1

Figure 1

CombinedMMS22LandCDAN1haplo‐insufficiency alters in vivo erythropoiesis. (A) Electropherograms of the patient, her brother, and son showing Sanger sequence validation of the MMS22L Q732X and CDAN1 R725W mutations. Arrows indicate the position of the nucleotide's substitutions. (B) Quantification of the o‐dianisidine positive area in the yolk sac of each embryo injected with the indicated morpholinos. Analysis was performed using the ImageJ software. Data are representative of two independent experiments. (C) Representative images of 48 hpf o–dianisidine–stained embryos injected with the indicated morpholinos. MOs used in this experience result in a 50% downregulation of gene expression. P‐values are determined by a two‐tailed t‐test. ns: not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.