Locomotor behavior analysis of 120 hpf zebrafish larvae following 120 h exposure to TAN and n-TiO2: (a) mean swimming velocity (cm/s) recorded at 60-s intervals during light–dark transition stimulation. The horizontal axis shows dark phases (black bars, 25–30 min) and light phases (white bars, 20–25 min and 30–35 min); (b) average swimming speed (cm/s) calculated per 5 min photoperiod interval, summarizing locomotor activity during each light or dark phase; (c) representative movement trajectories of larvae during the dark phase (red curve); * indicates significant differences between the control and exposed groups (p < 0.05); # indicates significant difference between TAN and n-TiO2 + TAN treatments (p < 0.05). Note: TAN L: low-concentration TAN; TAN M: medium-concentration TAN; TAN H: high-concentration TAN.
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