ZFIN Image: Stonkus et al., 2025, Figure 5.

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Figure Caption

Figure 5.

miRNA 3′-terminal demethylation assay. Sodium periodate (NaIO₄)-mediated oxidation followed by β-elimination (A) shows that human PHYHD1 and plant PHYH-like demethylases do not remove the 2′-O-methyl group from the miR173/miR173*^CH3 duplex (B). Solid arrows indicate full-length RNA strands. The dotted arrows point to the position of the unmethylated strand truncated by β-elimination. Reaction mixtures contained 0.1 μM RNA duplex, 5 or 100 μM oxygenase, 10 mM 2-oxoglutarate, 1 mM ascorbate, and 0.25 mM FeSO₄ in 50 mM Tris–HCl, pH 8.0. Incubation was performed for 4 h at 30°C, followed by periodate treatment.

Acknowledgments

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