Fig. 4 Sfrp1a impacts lymphoid differentiation in a dose-dependent manner. A. Representative images of uninjected AB∗ embryos or injected sfrp1a ATG MO embryos, fixed at 4 dpf, and analyzed by WISH for rag1. Scale bar = 50 μm. B. Quantification of A. C. Representative images of AB∗ embryos injected with 50 pg control mRNA or 50 pg sfrp1a mRNA, fixed at 4 dpf, and analyzed by WISH for rag1. Scale bar = 50 μm. D. Quantification of C. E. Representative images of AB∗ embryos injected with 500 pg control mRNA or 500 pg sfrp1a mRNA, fixed at 4 dpf, and analyzed by WISH for rag1. Scale bar = 50 μm. F. Quantification of E.
Reprinted from Developmental Biology, , Ide, A.D., Carpenter, K.A., Elaswad, M.T., Opria, K., Marcellin, K., Gilliland, C., Grainger, S., Secreted Frizzled-Related Protein 1a regulates hematopoietic development in a dose-dependent manner, , Copyright (2025) with permission from Elsevier. Full text @ Dev. Biol.