Figure 3

Figure 3 Telomerase deficient zebrafish exhibit C-circles and heterogeneous telomere length in regenerative tissue.

(A) C-circles abundance in regenerative tissue from wild type and telomerase-deficient adult zebrafish at 0, 24, and 48 hpa. n = 3 experiments. Each dot represents a biological replicate. (B) Representative images of Q-FISH in blastema cells from tert^+/+ and tert^−/− genotypes at time 0 dpa and 7 dpa. Red staining shows telomeres and blue represents nuclei staining. Scale bar, 16,3 μm. (C) Telomeric fluorescence values (arbitrary units of fluorescence, AUF) of regenerative cells from tert^+/+ and tert^−/− adult zebrafish at two times. n = 1000 cell events. (D) Telomeric fluorescence frequency of blastema cells from tert^+/+ and tert^−/− adult zebrafish at 0 dpa and 7 dpa. Very long telomeres have a higher fluorescence of 200,000 AUF, and very short telomeres have a lower fluorescence of 30,000 AUF. (E) Schematic representation of the behaviour of very long (blue arrow) and very short telomeres (black arrow) during fin regeneration of wild type and tert^−/− fish. All data were mean + s.e.m. n.s. not significant. ****P < 0.0001 for, one-way ANOVA plus Dunnett’s post hoc test (A) and Student t-test (C, D). When exact p values are not indicated is because GraphPad Prism software does not show it. Source data are available online for this figure.