Figure 2

Figure 2 Telomere length is maintained during consecutive amputations in telomerase-deficient zebrafish.

(A) Experimental design of a regeneration assay with 12 consecutive amputations every 7 days and growth monitoring (arrowhead and line), in tert^+/+, tert^+/−, and tert^−/− genotypes, at 4 and 11 months of age. The caudal peduncle is indicated in a blue dotted line. (B) Slopes of the regeneration areas after 12 consecutive clips in the three telomerase genotypes at 4 months of age. n = 3 experiments with 2 biological replicates each. (C) Slopes of the regeneration areas after 9 consecutive clips in the three telomerase genotypes at 11 months age. n = 3 experiments with two biological replicates each. (D) Measurement of telomere length by flow-FISH of regenerative fin tissue cells in all three telomerase genotypes at 4 months of age n = 2 experiments with 1 biological replicate. (E) Measurement of telomere length by Flow-FISH of regenerative fin tissue cells in all three telomerase genotypes at 11 months of age. n = 2 experiments with 1 biological replicate. All data were mean + standard deviation. ***P < 0.001 and ****P < 0.0001 for two-way ANOVA plus Dunnett’s post hoc test, calculated using data at clip number 9. When exact p values are not indicated is because GraphPad Prism software does not show it. Source data are available online for this figure.