Figure 3.

Figure 3.

Lipid droplet accumulation in siRPGR^ORF15 RPE-1 cells. (A) The protein level of Perilipin1, RPGR^ORF15, and RPGR^ex1-19 in NC, and siRPGR^ORF15 RPE-1 cells were detected and quantified (B). The * may indicate an alternatively spliced or post-translationally modified isoform (n = 3 biological replicates). (C) Nile red staining and TG concentrations (D) were measured in both NC and siRPGR^ORF15 RPE-1 cells, with arrows highlighting the LD. Scale bar = 20 µm (n = 3 biological replicates). (E) Filipin staining and FC concentrations (F) were measured in NC and siRPGR^ORF15 RPE-1 cells. The white arrows mark the FC. Scale bar = 10 µm (n = 3 biological replicates). Statistical significance is indicated as not significant (ns), **P < 0.01, ***P < 0.001.