Figure 5

Figure 5

In vivo evaluation of the anti-inflammation and antioxidant effect of resveratrol-derived polyphenols in the spint1a−/− inflammaging model. (A) Representative images of lyz:dsRed2 in spint1a knockout zebrafish larvae from each treatment group. (B) Quantification of total neutrophils counts. (C) Quantification of neutrophils located outside the caudal hematopoietic tissue (CHT) within the indicated region of interest (ROI, discontinuous white squares) after 2 days of treatment. Neutrophils are labeled in red. (D) Representative images of ROS staining in spint1a−/− larvae from each treatment group. (E) Quantification of the cellular oxidative stress levels in the tail after 2 days of treatment. (F) Representative images of acridine orange (AO) staining in spint1a−/− larvae from each treatment group. (G) Quantification of cell death levels in the tail after 2 days of treatment. Violin plots display the distribution with the media indicated by a red horizontal line, overlaid with raw data points representing individual larvae from two to four independent experiments (N = 2–4). Statistical significance: * p < 0.05; ** p < 0.01; **** p < 0.0001, determined by one-way ANOVA followed by Dunnett’s multiple comparison test. Scale bar: 500 μm.