Figure 4

Figure 4

In vivo evaluation of the telomeric effect of resveratrol-derived polyphenols in the prematurely aged ST2 model. (A) tert mRNA levels were quantified in 3-dpf zebrafish larvae by real-time RT-qPCR and normalized to rps11. (B) Telomerase activity was measured in 3-dpf zebrafish larvae using 0.5 µg of protein extract. (C) Telomere length was assessed in 3-dpf zebrafish larvae by qPCR using 60 ng of gDNA and expressed as telomere content relative to the single-copy gene rps11. Bars represent the mean ± SD of 3 independent experiments (N = 3) with 20–25 pooled larvae each (n = 20–25), relative to DMSO control. * p < 0.05, ** p < 0.01, **** p < 0.0001, determined by one-way ANOVA followed by uncorrected Fisher’s LSD test. (D) Kaplan–Meier survival curves of ST2 larvae under different treatments. (E) Summary of survival metrics. The table indicates sample size (in brackets), survival duration (days), and associated p-values for each treatment condition.