Figure 5

Figure 5 ARHGAP19 variants have defective GAP activity and cell migration.

(A) In vitro GAP activity assay measuring the GTPase rate of RhoA in the absence (intrinsic) or the presence of GAP domain from ARHGAP19 WT or mutants. Data are presented as means ± SEM from 3 independent experiments (n = 3; ****P < 0.0001; 1-way ANOVA) (B) MTT assays in fibroblasts from healthy controls (wt#223 and wt#1419) and ARHGAP19 mutants (n = 3). (C and D) Fibroblast cells were subjected to the Boyden chamber migration assay (C) or wound healing scratch assay as described in Methods (D) (n = 4). Representative images of migrated cells in the Boyden chamber assay are shown in C. Data are presented as mean ± SEM (*P < 0.05, **P < 0.01; 1-way ANOVA).