FIGURE 2

FIGURE 2

Survival and phenotypic characterization of the pex1 mutant zebrafish line. (A) Survival curves up to 15 days post-fertilization (dpf) (n = 50). (B) Percentage of wild-type (WT), heterozygous (pex1^+/–), and homozygous (pex1^–/–) mutant offspring reaching adulthood from F2 to F4 generations. (C) For organ morphology characterization, ten and five animals per genotype were dissected at 4 and 10 months, respectively. Representative post-mortem images of WT and pex1^–/– female and male fish showing hepatic steatosis already at 4 months. Example of degenerated ovaries [O] and enlarged fatty liver in a 10-month-old female. Testis are indicated by a [T] in 10-month-old males. (D) H&E staining of liver sections from 7-month-old female fish revealed vacuolization in pex1^–/– samples (the images are representative of 2 biological replicates). Scale bars represent 100 μm in (C,D).