ZFIN Image: Choudhury et al., 2025, Figure 2.

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Figure 2.

WT and (p)ppGpp⁰ mutant both replicate in vivo. Growth of Staphylococcus aureus WT and (p)ppGpp⁰ in (A) live and (B) dead zebrafish larvae after injection of 3000–4000 CFU (dotted line) into the bloodstream at 30 hours postfertilization. Zebrafish, 60–70, were injected with each strain, with 5 live larvae and any dead larvae taken at the specified time points for CFU/embryo determination. Survival was monitored until 93 hpi when the larvae reached 5.2 days postfertilization. The experiment was performed in biological triplicate with all 3 plotted. Abbreviations: (p)ppGpp⁰, guanosine tetraphosphate/guanosine pentaphosphate null mutant; CFU, colony-forming unit; hpi, hours postinfection; WT, wild type.

Acknowledgments

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