ZFIN Image: Shinkai et al., 2025, FIGURE 3

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FIGURE 3

Effects of pre-test stress loading on the outcomes of the NTT. Values of latency to enter the top half (LTTH) (A), time spent in the top half (TSTH) (B), and frequency of entries (FE) (C) during the NTT under combinations of light/dark conditions and no-restraint/restraint stress. Although no significant differences were observed in mean LTTH across all conditions, variability was significantly reduced (p < 0.05, F-test) under restraint compared with that in the control under both light and dark conditions. The same individuals (n = 10; 4 males and 6 females) were used across all conditions.

Acknowledgments

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