Figure 9

Figure 9

Behavioral analyses of larvae injected with either WT or MUT human AP1G1 mRNAs and compare to not injected embryos (CTRL). (A) Time course of the distance traveled by the larvae (6 days post-fertilization) overexpressing either WT or MUT human AP1G1 mRNAs and not injected embryos during 120 min of observation, with the first 30 min spent in the light (habituation phase). Each data point represents the mean (±SEM) of all different subjects (WT AP1G1 mRNA n = 50, MUT AP1G1 mRNA n = 48; CTRL n = 50). The experiment was performed in triplicate. Locomotor activity was quantified as the total distance traveled by the larvae (cm) (B) and their velocity (mm/s). (C) The Results in (B,C) are expressed as violin plot distributions: inside each violin, the central dashed line marks the median (Q2), and the superior and inferior dotted lines mark the third (Q3) and first (Q1) quartiles, respectively. The raw data plot represents the mean ± SD of the distance traveled by embryos in 2 min time bins of three experiments performed with the Noldus Ethovision software (XT 13.0 version). Data from independent experiments were analyzed using one-way ANOVA with Tukey’s multiple comparison test: * p < 0.005; **** p < 0.0001; ns = not significant.