Figure 8

Figure 8

Hatching and touch-evoked tests to assess locomotor responses. Zebrafish embryos were injected with human WT and MUT AP1G1 mRNA, and hatching rate and touch-evoked tests were performed at 72 and 96 hpf, respectively. Three independent replicates were performed, with 30 embryos for each experiment and treatment (90 embryos per condition). Non-injected embryos were used as controls (CTRL). (A) Percentage of embryos hatched at 72 hpf. (B) Embryos were injected with human WT and MUT AP1G1 mRNAs and maintained at 28 °C until 96 hpf. After gently touching the tail of each embryo, the touch swimming response was observed and categorized as follows: embryos that did not move, those that swam < 20 mm within the motility wheel, and those that swam > 20 mm in the wheel. Percentages of each category for each mutant were then calculated, considering 30 embryos for each treatment and each of the three experiments, and plotted (C) Schematic representation of the motility wheel used for the touch-evoked test.