Morphological phenotypes of embryos injected with human WT and MUT AP1G1 mRNA. (A) Representative embryos at 24, 48, 72, and 144 hpf developmental stages showing the main morphological effects after the injection of human WT and MUT AP1G1 mRNAs. Experiments were conducted three times, and 40 embryos were injected with either mRNAs. Uninjected embryos were used as controls (CTRL group). All images are lateral views with the anterior to the left (magnification 20×). (B) 144 hpf. Arrow indicates edema. Abbreviations: f, forebrain; mhb, midbrain–hindbrain boundary; hb, hindbrain; nt, notochord that are indicated by arrows in Figure 7A.
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