Fig. 3

Fig. 3

SMIM45-107aa promoted HCC cell proliferation, migration and tumor formation. A Schematic diagram of the SMIM45-107aa-Flag fusion constructs. The start codon of SMIM45-107aa was mutated to ATT. B SMIM45-107aa expression was analyzed in HCC cell lines. C–D Stable cell lines were established, and SMIM45-107aa overexpression or knockdown was detected by Western blot. E–F Wound-healing assays were performed to assess cell migration in SMIM45-107aa-overexpression SK-Hep1 andor QGY-7701 cells compared with negative controls (n = 3). Scale bars, 50 μm. G–H Colony formation assays were performed in SMIM45-107aa-overexpression SK-Hep1 andor QGY-7701 cells compared with negative controls (n = 3). I–J Wound-healing assays were performed to assess cell migration in SMIM45-107aa-knockdown SNU449 andor QGY-7701 cells compared with negative controls (n = 3). Scale bars, 50 μm. K–L Colony formation assays were performed in SMIM45-107aa-knockdown SNU449 andor QGY-7701 cells compared with negative controls (n = 3). M Tumor formation was evaluated in nude mice injected with SK-Hep1 cells stably overexpressing SMIM45-107aa andor control cells. N–O Tumor volume and weight were analyzed in the two groups (n = 4). (*p < 0.05; **p < 0.01). OE, overexpression; Mut, mutation; EM, empty vector