ZFIN Image: An et al., 2025, Fig. 1

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Figure Caption

Fig. 1

LINC00634 encoded a novel peptide (A) Differentially expressed genes of HCC were analyzed using data from TCGA. (B) Survival data of HCC patients were analyzed based on TCGA. (C) A Venn diagram was used to illustrate the potential coding ability of lncRNAs in HCC, based on the overlap between TCGA, Ribo-seq, and lncPEP database. (D) The coding ability of 16 lncRNAs was predicted using the online tools CPC2 and CPAT. (E) The ORF sequence of LINC00634 was predicted by ORFFinder. (F) The coding ability of LINC00634 was predicted using CPC2. (G) The ORF3-Flag fusion plasmid (pcDNA3.1-Flag) was transfected into HEK-293T cells, and protein expression was detected by Western blot. (H) The ORF3-Flag fusion plasmid (pcDNA3.1-Flag) was transfected into QGY-7701 cells, and the Flag-fusion protein location (green) was detected using IFC assay. Scale bars, 75 μm

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