SMIM45-107aa-derived short peptides inhibited HCC cell migration in vitro. A Schematic diagram of 7 peptides derived from SMIM45-107aa. B Biotin pull‑down assays were performed to assess the binding of 7 peptides to MTDH. C-D The effect of 7 peptides on cell migration was evaluated using wound-healing assays in SK-Hep1 cells transfected with an empty vector and in SMIM45-107aa-overexpression SK-Hep1 cells. Scale bars, 50 μm. E The effect of peptide 5 on cell migration was evaluated using wound-healing assays in QGY-7701. Scale bars, 50 μm. F IFC experiment detected the location of peptide 5 in QGY-7701 cells using biotin labeling. Scale bars, 70 μm. G Detection of cell colocalization of overexpressed SMIM45-107aa in SK-Hep1 cells treated with peptide 5 by IFC, and the control peptide was control group. Scale bars, 70 μm
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