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Fig. 5 The promoters of mitochondrial and heme-related genes have light- and ROS-responsive modules. (A–C) Above: Bioinformatic prediction of D-box (blue) and E-box (yellow) sequences in the promoters of the hebp2 (A), abcb6a (B), and soul5 (C) genes. Exons, Transcription Start Sites (TSS), and start codons (ATG) are indicated for each promoter fragment. The upper bars indicate the fragments cloned in luciferase reporter vectors and their respective lengths in base pairs (bp). Distances from the ATG are also indicated. Middle: Representative (of 3) in vivo bioluminescence assays in PAC-2 (gray) and EPA (black) cells transfected with the hebp2-Luc (A), abcb6a-Luc (B), and soul5-Luc (C) luciferase reporters exposed to 24 h of darkness followed by LD cycles. Below: Representative (of 3) in vivo bioluminescence assays in PAC-2 cells treated with H2O2 after transfection with the hebp2-Luc (A), abcb6a-Luc (B), and soul5-Luc (C) luciferase reporters and kept in complete darkness. Traces from cells treated with 1 mM H2O2 at 0 h (blue arrow) are plotted in light blue; untreated controls are plotted in gray.