ZFIN Image: Hall et al., 2025, Figure 1.

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Figure Caption

Figure 1.

Characterization of the newly engineered mgat1a−/− zebrafish line. N-glycan types and the sequential processing initiated by GnT-I (mgat1) from oligomannose-type to hybrid-type and conversion of the latter type to complex-type via GnT-II (A). The dashed green box encloses the common pentasaccharide core. Blue, red, and green symbols denote GlcNAc, Fuc, and Man, residues, respectively. N represents Asn residue of an N-glycosylated protein. Agarose gel of genomic DNA fragment digested with BclI from heterozygous and homozygous fish raised from crossing mgat1a+/− fish with indel and Wt alleles (B). DNA Chromatogram of a fragment of mgat1a from a Wt AB fish compared to that of the mgat1a−/− fish, revealing thirteen nucleotide deletions (∆13) (C).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int J Transl Med (Basel)