Figure 5

Figure 5

Overexpression of miR-96 and miR-182 leads to defects in endoderm cell convergence. (A–C) Fluorescence image of the endoderm of 36 hpf Tg (sox17: eGFP) transgenic zebrafish embryo (ventral view). Control embryos display normal endoderm cell convergence (A); both miR-96 OE (B) and miR-182 OE (C) embryos exhibit endodermal defects with holes in the central (white asterisks). (D,E) Quantification of endodermal defects in miR-96 OE (D) and miR-182 OE (E) embryos. (F) sox32 mRNA expression levels were analyzed by RT-qPCR in control, miR-96 OE, and miR-182 OE embryos at 36 hpf. (G–G”) WISH detected the expression of sox32 in control (G), miR-96 OE (G’), and miR-182 OE (G”) embryos at 9 hpf (ventral view; red arrow heads indicate the kupffer’s vesicle). The numbers in the lower right corner indicate the number of embryos. ** p < 0.01.