ZFIN Image: Zeng et al., 2025, Figure 3

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Figure Caption

Figure 3

Overexpression of miR-183 disrupts endoderm convergence movement. (A,B) Fluorescence image of the endoderm of transgenic zebrafish embryos with Tg (sox17: GFP) at 36 hpf (ventral view). (A) Control embryos show normal endoderm cell convergence. (B) miR-183 OE embryos exhibit endodermal defects with holes in the central region of the tissue (indicated by white asterisk). Scale bar: 500 μm. (C) Quantification of endodermal convergence defects in miR-183 OE embryos. (D) sox32 mRNA expression levels were analyzed by RT-qPCR in wild-type and miR-183 OE embryos at 36 hpf. (E,E’) WISH detecting sox32 expression in control and miR-183 OE embryos at 9 hpf (ventral view; red arrow heads indicate the kupffer’s vesicle). The numbers in the lower right corner indicate the number of embryos. ** p < 0.01.

Acknowledgments

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