ZFIN Image: Kumari et al., 2025, Figure 6

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Figure 6

Histological analysis of Danio rerio tissues under the effects of CaO-NP-enriched diet: (A) Liver tissue from the negative control group showing normal anatomy. (B) Liver tissue from the T1 group (2.4 mg Cao-NPs/kg feed) showing pyknosis, degenerated hepatocytes, and numerous vacuoles, indicating severe damage and metabolic dysfunction (Note: H—hepatocyte, LVH—liver vacuolation hepatocyte, KLC—Kupffer-like cell; scale bar: 50 µm, magnification: ×400). (C) Intestinal tissue from the negative control group with normal villi and goblet cells. (D) Intestinal tissue from the T1 group (2.4 mg Cao-NPs/kg feed) showing vacuolization of enterocytes, hypertrophy of smooth muscles, pseudo crypts, and muscle layer degeneration, along with damaged goblet cells (Note: GC—goblet cell, FV—fused villi, VE—villar epithelium, N—necrosis, V—villi; scale bar: 100 µm, magnification: ×200).

Acknowledgments

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