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Fig. 1

Low concentrations of HMH have no cytotoxicity. RAW 264.7 macrophages (1 × 105 cells/mL) were treated with 0–100 mg/mL HMH for 24 h. (A) The relative cell viability was measured using the MTT assay and compared to the untreated cells. (B) Representative images of the cells were captured using phase-contrast microscopy (× 20). Scale bar = 20 μm. (C) In a parallel experiment using flow cytometry, cell viability (D) and total cell count (E) were measured using a Muse Count & Viability Kit. ∗∗∗p < 0.001 vs. untreated cells.

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