Figure 8

Figure 8 There are fewer paired Cav1.3‐Rib b puncta in kif1aa mutants

A,B, example immunostain of neuromasts at 5 dpf in kif1aa mutants (B) or sibling control (A). Ribeye b (Rib b) labels ribbons or presynapses, and Ca_V1.3 labels calcium channels. Insets to the side show high magnification images of individual synapses. C, the number of paired Rib b‐Ca_V1.3 puncta is significantly reduced in kif1aa mutants compared with sibling controls (control: 4.13 ± 0.39; kif1aa: 3.59 ± 0.41; n = 13 control and 9 kif1aa neuromasts, unpaired t test, P = 0.00550). D, the average size of Ca_V1.3 puncta was similar between sibling controls and kif1aa mutants (control: 0.14 ± 0.02; kif1aa: 0.15 ± 0.02, unpaired t test, P = 0.0953). E–F, the mean intensity and integrated intensity of Ca_V1.3 puncta were significantly higher in kif1aa mutants compared with controls (E, mean intensity, control: 1685 ± 459; kif1aa: 2961 ± 1002, unpaired t test, P = 0.000600; F, integrated intensity, control: 230 ± 73.6; kif1aa: 444 ± 180, unpaired t test, P = 0.0009). n = 13 control and 9 kif1aa neuromasts in C–F. Scale bars in A = 5 µm, inset is 0.5 µm.