Figure 3

Figure 3 Kif1aa mutants have fewer ribbon synapses

A,B, example immunolabel of neuromasts at 5 dpf in a kif1aa mutant (B) or sibling control (A). Myosin7a (Myo7a) labels hair cells, Ribeye b (Rib b) labels ribbons or presynapses, and MAGUK labels postsynapses. C, the number of hair cells per neuromast is the same in kif1aa mutants compared with sibling controls (control: 15.6 ± 1.3; kif1aa: 16.0 ± 1.7, unpaired t test, P = 0.399). D, Kif1aa mutants have fewer complete synapses per cell (control: 3.13 ± 0.35; kif1aa: 2.68 ± 0.56, unpaired t test, P = 0.00870). E, in kif1aa mutants, the average area of Rib b puncta (presynaptic) was similar to sibling controls (control: 0.21 µm² ± 0.02; kif1aa: 0.20 µm² ± 0.02, unpaired t test, P = 0.345). F, in kif1aa mutants, the average area of MAGUK puncta (postsynaptic) was slightly larger compared with sibling controls (control: 0.19 µm² ± 0.02; kif1aa: 0.20 µm² ± 0.02, unpaired t test, P = 0.0498). n = 18 control and 15 kif1aa neuromasts in C–F. Scale bar in A = 5 µm.