Figure 3

Figure 3

mRNA expression of sgms2a and sgms2b in osteogenic cell subpopulations during cranial vault development in WT juvenile zebrafish by single-cell RNA sequencing. (A) UMAP analysis of cranial vault pooled cells from WT zebrafish and (n = 9509), with the cell type assignments for clusters. (B) The feature plots for sgms2a and sgms2b. The color intensity corresponds to the mean normalized expression level. (C) UMAP pictures representing osteogenic (OC) cells (including osteoprogenitors (OP0 to OPB5) and osteoblasts (OB)) and chondrogenic (CH) clusters (n = 6096). (D) The feature plot for sgms2a in OC and CH cells. sgms2b expression was not detected. The color intensity corresponds to the mean normalized expression level. (E) A dot plot showing sgms2a and sgms2b mRNA expression in OC and CH clusters. The dot diameter corresponds to the fraction of cells expressing each gene in each cluster, and the scale color corresponds to the mean normalized expression level. (F) UMAP analysis of pooled cells from WT zebrafish from the OB cluster (n = 605). (G) A dot plot showing sgms2a, sp7, sparc, and bglap mRNA expression in OB clusters. The dot diameter corresponds to the proportion of cells expressing each gene in each cluster, as shown on the scale. The color corresponds to the mean normalized expression level.