FIGURE 6

FIGURE 6

Single‐cell transcriptomic analysis of intestinal cell populations in wildtype, ret mutant, and DEAB‐treated zebrafish. (A) Uniform manifold approximation and projection (UMAP) analysis of scRNA‐seq showing nine distinct cell types. UMAP atlas was separated between wildtype, ret mutant, and DEAB‐treated zebrafish cells. (B) Bar plot (top panel) and stacked bar plot (bottom panel) showing the cell number contributing to each subcluster of all intestinal cells in wildtype (light‐blue), ret mutant (light‐purple) and DEAB‐treated zebrafish (pink) samples. (C) Feature dot plot representing expression levels of selected genes per subcluster between wildtype, ret mutant, and DEAB‐treated zebrafish cells. Expression levels within the dot plot are color‐coded (Pink: Up‐regulated; green: Downregulated, in each cell type compared to the rest other cell types). Gene expressed percentages in each cell type among wildtype, ret mutant, and DEAB‐treated zebrafish are indicated by dot sizes.