Fig. 5

Fig. 5 Effect of CAV-1 on Aibp2-regulated LEC specification.

a Representative TEM images of control and cav1^−/− zebrafish. Arrows show typical caveolae in cardinal vein ECs. b Quantification of caveolae in a. n = 27 (control) and n = 20 (cav1^−/−) cells. c Maxi-projection confocal images of Prox1⁺ cells in the 36 hpf apoa1bp^−/−; fli1a:egfp zebrafish with cav-1 crRNA alone or cav1-crRNA and Cas12a protein co-administration. Arrows show the Prox1⁺ LECs in cardinal vein. Green marks blood vessels, and red marks Prox1 staining. d Quantitative data of Prox1⁺ LECs in cardinal vein (7 somites) of (c). n = 34 embryos in both groups. e Representative confocal images of LEC specification in the cardinal veins of Vegfr3^AAA knock-in zebrafish at 36 hpf. Green marks blood vessels, and red marks Prox1+ cells. f Quantitative data of LEC numbers in (e). n = 13 (flt4^+/+) and n = 11 (flt4^+/AAA) embryos. g Confocal imaging of Prox1⁺ LECs in the indicated genetically modified fli1a:egfp zebrafish at 36 hpf. Green marks blood vessels, and red marks Prox1 staining. h Quantitative data of Prox1⁺ LECs of 7 somites in (g). n = 22 (apoa1bp^−/−; flt4^+/+) and n = 26 (apoa1bp^−/−; flt4^+/AAA) embryos. Mean ± SE in b, d, f, and h; unpaired two-sided t-test with Welch’s correction. CV cardinal vein. Scale bar: 500 nm in a; 50 µm in c, e, g. Source data are provided as a Source Data file.