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Fig. 1 Structural and molecular characterization of ube3a mutant zebrafish. (a) Schematic representation of the zebrafish ube3a gene structure showing the 11 exons. The point mutation (T > A) is located in exon 3. (b) Genotyping results showing the WT and mutant alleles. The T > A mutation introduces a unique site difference, allowing the differentiation between WT and mutant alleles. (c) Quantitative real-time PCR (RT—qPCR) analysis of ube3a mRNA relative expression (normalized) at 2, 10 days post-fertilization (dpf) and in adult brain tissue (N = 3 for WT and mutant, each). A reduction in ube3a mRNA levels is observed in mutant fish at larval stages. (d) Western blot analysis of the adult brain. The data are presented as the mean ± SEM. ns: non-significant, *P < 0.05.