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Fig. 4 Endothelial cell proliferation can be directly observed in the intersegmental vessels. The trunk of WT zebrafish was studied from 26 to 30 hpf in 15-minute intervals using a light sheet microscope. Endothelial nuclei were labelled using the transgenic marker fli1a:nls-mCherry (red) whilst endothelial F-actin was labelled using fli1a:lifeAct-mClover (green). EC proliferation was defined as where one nucleus visibly divides into two (examples are shown and dividing cells are labelled with yellow arrows; compare upper left with upper right; compare lower left with lower right).
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