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Fig. 12 Distribution pattern of the slow muscle fibers at the 23 twisted #04 of the lab goldfish strain. (A). The whole body (A1) and the magnified view of the caudal region (A2) of the goldfish (#2023-0417-09-#04, 8.59 mm [SL], 22 dpf). (B–C). Different levels of the sections immunostained with the slow muscle fiber specific antibody (F59). The magnified views of the sections are indicated by dashed line boxes with panel labels. White asterisks indicate the ventral caudal skeleton complex including pural, hypural, and hemal spines. The muscular tissues located between the bifurcated caudal fin skeleton are indicated by black asterisks in panels C1, D1, C2, and D2. The black arrowhead in panel D3 indicates slow muscle fibers in the medial caudal muscle. Abbreviations: ad.c.v, adductor caudalis ventralis; f.c.d, flexor caudalis dorsals; ne, neural tube, no, notochord. Scale bars: A1 = 1 mm; A2, B2, C2, D1, D2 = 100 μm; D3 = 100 μm. The panels in the second row are at the same magnification.