Fig. 6

Fig. 6

miRNA-130b-3p overexpression inhibits osteogenic capacity of AIS patient primary osteoblasts through the IGF1/MAPK pathway.a. WB bands from AIS patient primary osteoblasts treated with NC Si, empty vector SiRNA; 130b Si, miRNA-130b-3p SiRNA; AXL1717, Picropodophyllin (n = 3) b. IGF1 expression levels are elevated in the 130b Si group and 130b Si + AXL1717 group c. IGF1R expression levels show no significant changes in the 130b Si group and 130b Si + AXL1717 group d. P-IGF1R expression levels are elevated in the 130b Si group e. Phosphorylation of ERK1/2 is elevated in the 130b Si group f. IGF1R expression levels remain unchanged in the 130b Si and 130b Si + AXL1717 groups g. RUNX2 expression levels are elevated in the 130b Si group h. OCN expression levels are elevated in the 130b Si group i. Osteogenic differentiation of primary osteoblasts in the 130b Si group shows improved osteogenic capacity j. Quantification of average optical density of Alizarin Red S staining(n = 3) ***:p<0.001; **:p<0.01; *:p<0.05