ZFIN Image: Li et al., 2025, Fig. 3

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Figure Caption

Fig. 3 EMB is required for ENCC proliferation and migration in vitro. A, Isolated and cultured ENCCs identified by immunofluorescence double labeling of p75 (green) and Nestin (red), the nuclei are labeled with DAPI (blue). B, Schematic overview of the lentiviral construct used for Emb gene knockout in ENCCs. C, Lentivirus infection is confirmed by GFP fluorescence at day 6. D, Relative mRNA expression level of Emb in neurospheres. E, Numbers of the neurospheres are reduced upon knockout of Emb. F, Average diameter of the neurospheres is reduced upon knockout of Emb. G, Cell growth curve in the proliferation assay showing that loss of Emb affects ENCC proliferation. H, The migration ability, accessed by numbers of cells passed the matrix in the transwell assay, is affected by loss of Emb. **, p < 0.01, ***, p < 0.001, one-way ANOVA with Bonferroni post-hoc, N = 6

Acknowledgments

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