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Fig. 3 MO knockdown of dennd5b indicates its critical role in embryo development. a An uninjected zebrafish embryo at 48 hpf exhibits no observable phenotype. b Zebrafish embryos injected with the standard control morpholino show no signs of physical deformity at 48 hpf. c–e A mild phenotype, f–h a moderate phenotype, and i–k a severe phenotype was observed following dennd5b MO knockdown, underscoring the critical role of dennd5b in early zebrafish embryonic development. The scale bar indicates 890 μm. l A graphical representation illustrates the effects of three different dosages of dennd5b MO injection, demonstrating a dose-dependent increase in phenotypic severity, including a compressed head and deformed tail, as compared to embryos injected with the standard control MO