|
Fig. 1 Multiple sequence alignments of dennd5b amino acid sequences. a The protein domains of zebrafish Dennd5b, including uDENN, cDENN, dDENN, RUN1, PLAT, and RUN2, are illustrated in a schematic representation. The gray line beneath the schematic denotes the positions of the amino acid residues, while the lighter gray line above the schematic marks the specific locations of the individual domains b The amino acid sequence alignment shows the high level of conservation of DENND5B between humans, chimpanzees, mice, and zebrafish. Amino acids highlighted in black shade indicate conservation of the residue between all proteins, those in grey reflect highly similar residues between proteins. Below the amino acid sequences, the blue line represents the position of the uDENN domain, while the light blue lines indicate the central DENN domain. The light pink line corresponds to the dDENN domain. The dark yellow lines denote the locations of the first RUN domain (RUN1), and the light yellow lines mark the second RUN domain (RUN2). Additionally, the green lines represent the PLAT domain, which separates the two RUN domains within DENND5B c Amino acid sequence alignments between zebrafish dennd5a and dennd5b, and light-yellow lines indicate the locations of the RUN2 domain in zebrafish dennd5b