Figure 4

Figure 4

Analysis of Adgrv1 expression in retinal sections of wild-type, adgrv1^rmc22, adgrv1^Δexon9, and adgrv1^Δexon40-42 zebrafish

(A) Schematic representation of a zebrafish photoreceptor cell. OS, outer segment; CC, connecting cilium; IS, inner segment; ONL, outer nuclear layer. (B) Retinal cryosections of wild-type, homozygous adgrv1^rmc22, adgrv1^Δexon9, and adgrv1^Δexon40-42 zebrafish larvae (5 dpf) labeled with antibodies directed against Adgrv1 (red) and centrin (green). Nuclei are counterstained with DAPI (blue). No Adgrv1 signal was detected in adgrv1^rmc22 and adgrv1^Δexon9 zebrafish retinae. In contrast, Adgrv1 was present and localized adjacent to centrin, a marker of the basal body and connecting cilium, in the retinas of wild-type and adgrv1^Δexon40-42 zebrafish larvae (n = 14 larvae per line, from two biological replicates for wild-type, adgrv1^rmc22, adgrv1^Δexon9, and adgrv1^Δexon40-42 larvae). Magnified images are shown at the right side. Scale bars, 10 μm.