ZFIN Image: Chugh et al., 2025, Figure 2.

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Figure Caption

Figure 2.

Characterization of NK cell mimics: (a) surface charge on cG, CM and cGCM, N = 3, (b) Fourier transform infrared (FT-IR) spectra of cG, CM, and cGCM, (c) SDS-PAGE gel stained with coomassie brilliant blue dye to assess the protein profile on cG, CM and cGCM, (d) Protein loading yield (%) on cGCM, N = 3, (e) Western blot to identify specific protein (NKp30, NKG2D, DNAM-1) on CL, CM and cGCM, and (f) Protein absorption analysis on cG and cGCM after incubation in FBS for 3 h at 37°C (cG vs cG_FBS, p < 0.0001, 95% CI = −3.004 to −2.355), N = 3.

Abbreviations: cG: cross-linked gelatin microspheres; cG_FBS: cross-linked gelatin microspheres incubated in foetal bovine serum; cGCM_FBS: NK cell mimics incubated in foetal bovine serum; CL: KHYG-1 cell lysate; CM: isolated KHYG-1 cell membrane; cGCM: NK cell mimics; MW: molecular weight.

Graphs are plotted in box and whiskers format with max and min value showing all data points.

*p < 0.05. **p < 0.01. ***p < 0.001. ****p < 0.0001.

Acknowledgments

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