Figure 3

Figure 3

Design and characterization of the genetically modified adgrv1^Δexon9 and adgrv1^Δexon40-42 zebrafish lines

(A) Schematic representation illustrating the exon-excision approach. The anticipated excision of the target exons in injected embryos (1 day post-fertilization [dpf]) was confirmed by Sanger sequencing analysis. Excision of the genomic segment encompassing adgrv1 exon 9 led to the incorporation of three additional nucleotides (GTT) at the repair site. (B) RT-PCR results demonstrate the absence of adgrv1 exon 9 in adgrv1^Δexon9 larvae, as well as the absence of exons 40–42 in adgrv1^Δexon40-42 larvae (5 dpf). Sanger sequencing of RT-PCR amplicons confirmed the successful removal of the target exons from the adgrv1 transcripts. PCR (−): negative PCR control.