Figure 9.

Figure 9.

Sialyltransferase inhibitor (STI, 3Fax-Peracetyl Neu5Ac) studies: (a) Release of STIanalysis from gelatin microspheres (cG) and NK cell mimics (cGCM) using a high-performance liquid chromatography (HPLC) system, N = 3, Effect of STI, STI loaded gelatin microspheres (cG_STI), and STI loaded NK cell mimics (cGCM_STI) on MDA-MB-231 cells. Mean fluorescence intensity/cell of (b) Sambucus nigra (SNA) lectin (C vs STI, p< 0.0001, 95% CI = 2458–1208; C vs cG_STI, p = 0.0015, 95% CI = 644 to 393.5; C vs cGCM_STI, p = 0.0011, 95% CI = 1686 to 436.2; cG vs cG_STI, p = 0.0363, 95% CI = 1286–35.53; cGCM vs cGCM_STI, p = 0.0228, 95% CI = 1337 to 86.87), (c) fluorescence microscopic images of sambucus nigra (SNA) lectin expression, (d) peanut agglutinin (PNA) lectin, (e) Maackia amurensis (MAA) lectin, and (f) qheat germ agglutinin (WGA) lectin expression after treatment of 200 μM STI, cG, cGCM, cG_STI, cGCM_STI for 72 h at 37°C, N = 3.

Abbreviations: C: Control (cells with only media), cG: gelatin microspheres, cGCM: NK cell mimics.

Note. No cytotoxic payload was incorporated in these experiments. Note: This study aimed to assess the controlled release of functional glycan modulators, not tumour cell killing or spheroid growth inhibition. Graphs are plotted in box and whiskers format with max and min value showing all data points.

*p < 0.05. **p < 0.01. ***p < 0.001. ****p < 0.0001.