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Fig. 9 Assessment of fertility and growth performance of amber and ruby mutants. (A) Fertilization rate of eggs from a wild-type female fertilized with sperms from mature male wild-type fish, amber mutants, and ruby mutants (n = 6 parent pairs/genotype). (B) Fertilization rate of eggs from mature female wild-type fish, amber mutants, and ruby mutants fertilized with sperms from a wild-type male (n = 6 parent pairs/genotype). (C) Fertilization rate of eggs from mature female wild-type fish, amber mutants, and ruby mutants fertilized with sperms from males of the same genotype (n = 6 parent pairs/genotype). (D) Survival rate of wild-type fish, amber, and ruby embryos (n = 6 parent pairs/genotype). (E) Malformation rate of wild-type fish, amber, and ruby embryos (n = 6 parent pairs/genotype). (F-I) Body length and body weight of wild-type fish, amber mutants, and ruby mutants at 90 dpf (n = 20 fish) and at 180 dpf (n = 12 fish).
Reprinted from New biotechnology, 89, Liang, G., Lu, B., Dai, S., Li, M., Yao, J., Liu, H., Liu, X., Liu, X., Wang, D., Creation of colorless transparent tilapia using CRISPR/Cas9 mediated multi-gene mutation, 163-176, Copyright (2025) with permission from Elsevier. Full text @ N. Biotechnol.