ZFIN Image: Zhang et al., 2025, FIGURE 4

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Figure Caption

FIGURE 4

Zebrafish genetic manipulation (A) Phylogenetic analysis of RAB11A in Zebrafish, Human, and Other Species. The phylogenetic tree was constructed using amino acid sequences of RAB11A homologs, generated with MEGA 12 via the Neighbor-Joining method (Bootstrap = 1000 replicates) and visualized using iTOL. Branch node values indicate Bootstrap support rates (shown if ≥ 50%). Scale bar represents amino acid substitutions per site. (B) Amino Acid Sequence Alignment of Key Functional Domains in RAB11A Homologs Across Species (C) Schematic of the rab11a gene (ENSDARG00000041450) and the red sequences represent sgRNA2, 3, 6. The bule part of exon 3 represents the sgRNA knockout target site. (D) The Sanger sequence of mutant rab11a edited by sgRNA2, 3, 6. (E) The procedure of the establishment of rab11a deficient zebrafish model.

Acknowledgments

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