Fig. 8 (-)-Terpinen-4-ol’s inhibition of RND efflux pumps in K. pneumoniae. Representative gene expression analysis of MDR K. pneumoniae BC1415 cells subjected to 1 h treatment with the following viz., erythromycin, commercial Terp-4-ol, Purified Terpinen − 4-ol and the positive control PAβN. RNA was extracted post treatment and equimolar concentration of RNA was converted to cDNA, and qRTPCR was performed. After normalisation with the housekeeping gene rpoB the gene expression levels were evaluated using ∆∆ct method and expressed as a relative fold-difference in Gene expression Commercial and Lactobacillus extracted (-)-Terpinen-4-ol is mentioned as Terp-4-ol and fractionated Terinen-4-ol as purified Terp respectively.
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