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Fig. 1 MYT1L mutation altered chromatin accessibility in murine hypothalamus. (A–B) Density plots (top) and genomic heatmaps (bottom) showing differential chromatin accessibility between WT (left) and MYT1L heterozygous (het) mutant mice (right) identified by ATAC-seq (FDR < 0.1) ranging from 3 kb upstream of transcriptional start site (TSS) and 3 kb downstream of transcriptional end site (TES). (C) Histogram displaying density of chromatin accessibility changes when comparing WT to MYT1L mutant het mice where increasing accessibility in het mice is represented by increasing log2FC values. (D–E) Dot plots showing results from monaLisa motif analysis where the size of the dot relates to the log2 enrichment score and the color represents –log10 adjusted p-value. Enrichment scores are arranged along the x-axis according to differential chromatin accessibility as in C. In accessible promoter regions (D) we see enrichment of general TF binding motifs while for differentially accessible enhancer regions (E) we see enrichment for neurogenic and activity-dependent TF binding motifs. N = 5 (3F and 2M) P60–70 mice per genotype.