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Fig. 3 Myt1l loss disrupted the number of AVP+ and OXT+ cell numbers in the paraventricular nucleus. (A–B) Immunofluorescence for nuclei (DAPI; blue), oxytocin (OXT; green), arginine vasopressin (AVP; red) in the PVN of the hypothalamus from WT mice (A) and Het mice (B), scale bar = 100uM. Images are representative of points closest to the average cell density by genotype. (C) Female and male Het mice have similar average PVN size (mm2) compared to WTs. (D–F) Het mice had significantly less AVP positive cells (D) yet significantly more OXT positive cells (E) compared to WT. (F) No significant interaction of genotype and sex was observed in AVP positive cells of the PVN. (G–H) Females had significantly less AVP positive cells compared to males (G) with no significant sex bias in OXT expressing cells (H). (I) No significant interaction of genotype and sex was observed in OXT positive cells of the PVN. Sample sizes are presented as points in all panels, averaging 3–5 sections per animal. Data are represented as mean ± SEM of animals with individual points presented as open circles. ANOVA used for statistical comparisons.