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Fig. 2 Transcriptional consequences of MYT1L mutation in murine hypothalamus. (A) Heatmap of significant (FDR < 0.1) gene expression changes in Het mice as compared to WT mice with red indicating a higher expression (or Log2FC) in Het mice and blue indicating a lower expression in Het mice. N = 6 (3M and 3F) P60–70 mice per genotype. (B–C) GO analysis of upregulated (B) and downregulated (C) genes in Hets sorted by –log10 adjusted p-value. (D–H) GSEA plots displaying enrichment scores for various categories of genes as they relate to genotype. Green lines indicate the running enrichment score (ES) while x-axis represents the ranked genes in the gene set. The normalized enrichment score (NES) and false discovery rate (FDR) are included in the top right corner of each graph. (I) Venn diagram displaying overlap between differentially accessible chromatin regions from ATAC-seq and differentially expressed genes from RNA-seq.