ZFIN Image: Peloggia et al., 2025, Fig. 3.

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Figure Caption

Fig. 3.

Differential expression of Notch reporter and ion channel genes in the three distinct subtypes of olfactory ionocytes. (A–A’’) HCR RNA-FISH for foxi3a (yellow) and trpv6 (magenta), combined with the Notch reporter tp1bglobin:EGFP (cyan) and DAPI stain (gray). (B–B’’) HCR RNA-FISH for slc9a3.2 (yellow) and trpv6 (magenta) with DAPI stain (gray). (C–C’’) HCR RNA-FISH for slc4a1b (magenta) combined with the Notch reporter tp1bglobin:EGFP (cyan) and DAPI stain (gray). The arrows indicate adjacent cells with EGFP and slc4a1b expression. (D–D’’) Maximum intensity projection of a HCR RNA-FISH for slc12a10.2 (yellow) and foxi3b (magenta) with DAPI stain (gray) shows solitary, NCC-like ionocytes in the olfactory epithelium. (E–E’’) HCR RNA-FISH for slc12a10.2 (yellow) and chrd (magenta) with DAPI stain (gray). (F–F’’) HCR RNA-FISH for slc12a10.2 (yellow) and hepacam2 (magenta) with DAPI stain (gray). Scale bars: A, B, C, D, E, F, 20 µm; A’’, B’’, C’’, D’’, E’’, F’’, 5 µm.

Acknowledgments

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